![]() We want to use magnification in the microscope objective range, ×5 and above, so our ability to use conventional macro lenses is limited. This subject is small so magnifications above 1:1 will be needed to reveal its detail, but it is significantly thick and three dimensional so we cannot simply put it on a slide, squash it with a coverslip and examine it with the microscope. Let us suppose we wish to take a highly detailed picture of the head and eyes of a dead fly. This site is primarily for those interested in all aspects of the microscope and microscopy rather than the world of macrophotography, but increasingly these two interests are overlapping and coalescing as macrophotographers tackle smaller subjects and higher magnifications. In principle any semi-transparent structures which lie above or below other similar structures, can create headaches in stacking.īefore moving to consideration of the choice of stacking software, it is worthwhile to mention stacking in high magnification macrophotography. ![]() There is no substitute for retouching in this case, and Zerene Stacker’s ability to retouch the output from single frames from the stack is a powerful help. The result can be loss of detail and semi-transparent hairs instead of solid ones. A notorious example is fine overlapping hairs, when the software finds it difficult to decide what is above and what below.
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